[DSMZ] PACADD-188

어스바이오는 DSMZ 전문 취급점으로서 모든 제품을 전문적으로 취급 및 공급하고 있습니다.
DSMZ의 Cell line을 소개드립니다.
 

 

Cell line: PACADD-188
DSMZ no.: ACC 753
Species: human (Homo sapiens)
Cell type: pancreas carcinoma
Origin: established in 2011 from the intraepithelial neoplasia of a 68-year-old woman with a ductal adenocarcinoma of the pancreas (stage classification pT3N1M0/G3)
Biosafety level: 1

DSMZ Cell Culture Data:
 

Morphology: epithelial-like cells growing adherently in monolayers in tight islands
Medium: 80% mixture of DMEM and Keratinocyte SFM (at 1:1) + 20% h.i. FBS
Subculture: seed out at ca. 1*10⁶ cells/80 cm² flask; split confluent culture 1:3 once a week using trypsin/EDTA for about 10 min. to detach cells
Incubation: at 37 °C with 5% CO₂
Doubling time: ca. 2-4 days
Harvest: cell harvest of ca. 8*10⁶ cells/175 cm²
Storage: frozen with 70% medium, 20% FBS, 10% DMSO​​​​​​​​​​​​​​
 

DSMZ Scientific Data:

Mycoplasma: negative in PCR assay​​​​​​​
Authenticity: STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.​​​​​​​
Viruses: PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
 

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